Welcome to `fba`’s documentation!¶

fba: a flexible and streamlined package for single-cell feature barcoding assays.

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Maintainability

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工欲善其事，必先利其器。—— 论语·卫灵公

Quickstart¶

Installation¶

$ pip install fba

Usage¶

$ fba

usage: fba [-h]  ...

Tools for single-cell feature barcoding analysis

optional arguments:
-h, --help         show this help message and exit

functions:

   extract         extract cell and feature barcodes
   map             map enriched transcripts
   filter          filter extracted barcodes
   count           count feature barcodes per cell
   demultiplex     demultiplex cells based on feature abundance
   qc              quality control of feature barcoding assay
   kallisto_wrapper
                   deploy kallisto/bustools for feature barcoding
                   quantification

Citation¶

Jialei Duan, Gary C Hon, FBA: feature barcoding analysis for single cell RNA-Seq, Bioinformatics, Volume 37, Issue 22, 15 November 2021, Pages 4266–4268. DOI: 10.1093/bioinformatics/btab375. PMID: 33999185.