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  • Installation
  • User Guide
  • Tutorials
    • CRISPR screening
      • 10k A375 Cells Transduced with (1) Non-Target and (1) Target sgRNA, Dual Indexed
      • CROP-seq; 1:1:1 Mixture of DNMT3B, MBD1, and TET2 Knockout Cell Lines (HEK293T)
      • Direct-capture Perturb-seq; CRISPRi-based Screen of Unfolded Protein Response (UPR) Using 3' sgRNA-CR1<sup>cs1</sup>
    • Cell surface protein labeling
      • CITE-seq; 8k Cord Blood Mononuclear Cells with 13 Antibodies
      • ASAP-seq; Multiplexed CRISPR Perturbations in Primary T Cells
      • 1k Human PBMCs Stained with a Panel of TotalSeq B Antibodies, Dual Indexed
    • ECCITE-seq
      • 6k Single-cell Multimodal Readout of NIH-3T3, MyLa, Sez4 and PBMCs
    • PHAGE-ATAC
      • PHAGE-ATAC; Anti-CD8 Phage Hashing Single-cell ATAC-seq Using CD8 T Cells from Four Human Donors
    • CellPlex
      • 10k 1:1 Mixture of Raji and Jurkat Cells Multiplexed, 2 CMOs
      • 30k Mouse E18 Combined Cortex, Hippocampus and Subventricular Zone Nuclei Multiplexed, 12 CMOs
    • Cell hashing
      • Peripheral Blood Mononuclear cells with 8 Antibodies
    • MULTI-seq
      • 15k HEK293 and 40k HMECs Multiplexed by Lipid- and Cholesterol-tagged Indices
    • Targeted transcript enrichment
      • Hodgkin’s Lymphoma, Dissociated Tumor: Targeted, Gene Signature Panel
    • Pseudo-bulk
      • 10k A375 Cells Transduced with (1) Non-Target and (1) Target sgRNA, Dual Indexed
      • 10k 1:1 Mixture of Raji and Jurkat Cells Multiplexed, 2 CMOs

Development

  • Changelog
  • Acknowledgements
  • GitHub Repository
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CRISPR screening¶

  • 10k A375 Cells Transduced with (1) Non-Target and (1) Target sgRNA, Dual Indexed

  • CROP-seq; 1:1:1 Mixture of DNMT3B, MBD1, and TET2 Knockout Cell Lines (HEK293T)

  • Direct-capture Perturb-seq; CRISPRi-based Screen of Unfolded Protein Response (UPR) Using 3’ sgRNA-CR1cs1

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10k A375 Cells Transduced with (1) Non-Target and (1) Target sgRNA
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