Tutorials

• CRISPR screening

  • 10k A375 Cells Transduced with (1) Non-Target and (1) Target sgRNA, Dual Indexed

  • CROP-seq; 1:1:1 Mixture of DNMT3B, MBD1, and TET2 Knockout Cell Lines (HEK293T)

  • Direct-capture Perturb-seq; CRISPRi-based Screen of Unfolded Protein Response (UPR) Using 3’ sgRNA-CR1^cs1

• Cell surface protein labeling

  • CITE-seq; 8k Cord Blood Mononuclear Cells with 13 Antibodies

  • ASAP-seq; Multiplexed CRISPR Perturbations in Primary T Cells

  • 1k Human PBMCs Stained with a Panel of TotalSeq B Antibodies, Dual Indexed

• ECCITE-seq

  • 6k Single-cell Multimodal Readout of NIH-3T3, MyLa, Sez4 and PBMCs

• PHAGE-ATAC

  • PHAGE-ATAC; Anti-CD8 Phage Hashing Single-cell ATAC-seq Using CD8 T Cells from Four Human Donors

• CellPlex

  • 10k 1:1 Mixture of Raji and Jurkat Cells Multiplexed, 2 CMOs

  • 30k Mouse E18 Combined Cortex, Hippocampus and Subventricular Zone Nuclei Multiplexed, 12 CMOs

• Cell hashing

  • Peripheral Blood Mononuclear Cells with 8 Antibodies

• MULTI-seq

  • 15k HEK293 and 40k HMECs Multiplexed by Lipid- and Cholesterol-tagged Indices

• Targeted transcript enrichment

  • Hodgkin’s Lymphoma, Dissociated Tumor: Targeted, Gene Signature Panel

• Pseudo-bulk

  • 10k A375 Cells Transduced with (1) Non-Target and (1) Target sgRNA, Dual Indexed

  • 10k 1:1 Mixture of Raji and Jurkat Cells Multiplexed, 2 CMOs